The Production of Aspartame
Aspartame is produced through fermentation by combining B. flavum and C. glutamicum with nutrients carbohydrates, vitamins, and amino acids. When adequate amino acids are available, they are separated and purified.
Next, phenylalanine is reacted with methanol to form L-phenylalanine methyl ester. Aspartic acid is also modified and then the two amino acids are combined, heated and cooled, which results in the formation of crystals. The crystals are reacted with acetic acid to create aspartame. After further steps of distilling and filtering, the final purified aspartame is produced.
The ingredients used in the production of aspartame are regularly checked to assure quality and safety, including the bacterial cultures. In addition, the physical and chemical properties of the finished product are checked, such as pH level, melting point and moisture content to ensure that it meets product standards.